;hnhb.bv ;Bruker DMX Avance, XwinNMR version ;for correlation of 15N with hb ;use hnhbref.bv as reference for quantitation ;use with hn(co)hb to get hb stereoassignments ;and chi-1 angles in proteins ;for use with 15N-only-labeled proteins ;Ref Archer et al., (1991) JMR 95,636-641 ;STATES-Tppi with presat ;for gradient version, see hnhbgr.bv ;implemented by Brian Volkman, ;volkman@nmrfam.wisc.edu ;last modified 10/5/95 ;BMRB Pulse Sequence Accession Number: 29 ; ;p1 1H 90 ;p2 15N 90 Hipower ;p31 15N low power ;d0 hb t1 ;d10 15N t2 ;d2=1/4JNH ;d3=27ms "d26=p2-p1" "d2=2.3m" "p21=0.5m" "p22=2m" "d11=50m" "d12=11m" "d3=27m" "d25=(p1*2)+(d10*2)" 1 ze 2 d11 do:f2 3 d12 d12 4 d12 d12*2 5 d12 d12 6 d12 pl2:f2 d12 pl9:f1 p18 ph0 ;presat d15 pl1:f1 ; *** Start Inept to 15N *** p1 ph0 p21 ph10 d2 (d26 p1*2 ph0):f1 (p2*2 ph1):f2 d2 p22 ph0 ;messerele spin H2o suppression 2u (p1 ph1):f1 (p2 ph2):f2 ;***Evolve long range N-Hb via HMQC + Hb t1*** d3 d25 ;p1*2 compensation pulse (p1 ph6):f1 d0 (p2*2 ph8):f2 d0 (p1 ph7):f1 d3 ;*** Evolve 15N chem shift *** d10 (p1*2 ph0):f1 d10 ;*** Reverse inept to 1H *** (d26 p1 ph0):f1 (p2 ph3):f2 d2 5u (d26 p1*2 ph4):f1 (p2*2 ph5):f2 d2 pl16:f2 5u (p1 ph0):f1 go=2 ph31 cpd2:f2 d11 do:f2 wr #0 if #0 ip6 zd lo to 3 times 2 d12 ip31 d12 ip31 id0 lo to 4 times l4 ;States-TPPI Hb d12 ip3 rd0 d12 ip3 d12 ip3 lo to 5 times 2 ;increment 3* because after evolution d12 ip31 d12 ip31 id10 lo to 6 times l6 ;States-TPPI 15N exit ph0=0 ph1=1 3 ph2=0 ph3=0 ph4=0 ph5=0 ph6=0 0 2 2 ph7=0 ph8=0 0 0 0 1 1 1 1 2 2 2 2 3 3 3 3 ph10=1 ph31=0 2 2 0 2 0 0 2 ph20=0