BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4753

Title: Average solution structure of d(TTGGCCAA)2 bound to Chromomycin-A3 and zinc   PubMed: 10801486

Authors: Gochin, M.

Citation: Gochin, M.. "A High Resolution Structure of a DNA-chromomycin-Co (II) Complex Determined from Pseudocontact Shifts in Nuclear Magnetic Resonance"  Structure Fold. Des. 8, 441-452 (2000).

Assembly members:
DNA octamer, polymer, 8 residues, Formula weight is not available
chromomycin, polymer, 6 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA octamer: TTGGCCAA
chromomycin: XXXXXX

Data sets:
Data typeCount
1H chemical shifts135
13C chemical shifts96
31P chemical shifts7

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1d(TTGGCCAA) DNA octamer 11
2d(TTGGCCAA) DNA octamer 21
3chromomycin-A3 12
4chromomycin-A3 22
5Zn(II) 13
6Zn(II) 23

Entities:

Entity 1, d(TTGGCCAA) DNA octamer 1 8 residues - Formula weight is not available

1   DTDTDGDGDCDCDADA

Entity 2, chromomycin-A3 1 6 residues - Formula weight is not available

1   1GL2GLDXB1ARDDADDA

Entity 3, Zn(II) 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: DNA octamer 2 mM; chromomycin 2 mM; ZINC (II) ION 2 mM; NaCl 100 mM; Na borate 10 mM

sample_cond_1: pH: 6; temperature: 298 K; ionic strength: 110 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
13C-1H HMQCsample_1not availablesample_cond_1

Software:

NMRPipe v1.7 - processing

Sparky v3.0 - data analysis

X-PLOR v3.0, 3.851 - refinement

FELIX v2.1 - data analysis

NMR spectrometers:

  • GE Omega 500 MHz

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