BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4749

Title: NMR structure of a DNA three-way junction

Authors: Thiviyanathan, Varatharasa; Luxon, B.; Leontis, Neocles; Donne, D.; Gorenstein, D.

Citation: Thiviyanathan, Varatharasa; Luxon, B.; Leontis, Neocles; Donne, D.; Gorenstein, D.. "Hybrid-Hybrid matrix structural refinement of a DNA three-way junction from 3D NOESY-NOES"  J. Biomol. NMR 14, 209-221 (1999).

Assembly members:
DNA Three Way Junction, polymer, 10 residues, Formula weight is not available
DNA Three Way Junction, polymer, 10 residues, Formula weight is not available
DNA Three Way Junction, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: unclassified   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA Three Way Junction: GCTGCCACCG
DNA Three Way Junction: CGGTGCGTCC
DNA Three Way Junction: GGACGTCGCAGC

Data sets:
Data typeCount
1H chemical shifts282

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1twj 11
2twj 22
3twj 33

Entities:

Entity 1, twj 1 10 residues - Formula weight is not available

1   DGDCDTDGDCDCDADCDCDG

Entity 2, twj 2 10 residues - Formula weight is not available

1   DCDGDGDTDGDCDGDTDCDC

Entity 3, twj 3 12 residues - Formula weight is not available

1   DGDGDADCDGDTDCDGDCDA
2   DGDC

Samples:

sample_1: DNA Three Way Junction 2 mM; DNA Three Way Junction 2 mM; DNA Three Way Junction 2 mM; EDTA 0.5 mM; sodium_phosphate 100 mM; MgCl2 10 mM; NaCl 100 mM

sample_cond_1: ionic strength: 100 mM; pH: 6.8; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
3D NOESY-NOESYsample_1not availablesample_cond_1

Software:

MORASS v2.5 - iterative matrix relaxation

NMR spectrometers:

  • Varian Unity plus 750 MHz

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