BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4734

Title: HMG-D complexed to a bulge DNA: an NMR study

Authors: Cerdan, Rachel; Payet, Dominique; Yang, Ji-Chun; Travers, Andrew; Neuhaus, David

Citation: Cerdan, Rachel; Payet, Dominique; Yang, Ji-Chun; Travers, Andrew; Neuhaus, David. "HMG-D complexed to a bulge DNA: an NMR study"  Protein Sci. ., .-. (2000).

Assembly members:
High Mobility Protein of Drosophila, polymer, 112 residues, Formula weight is not available
bulge DNA 12/14mer, polymer, 14 residues, Formula weight is not available
bulge DNA 12/14mer, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
High Mobility Protein of Drosophila: MSDKPKRPLSAYMLWLNSAR ESIKRENPGIKVTEVAKRGG ELWRAMKDKSEWEAKAAKAK DDYDRAVKEFEANGGSSAAN GGGAKKRAKPAKKVAKKSKK EESDEDDDDESE
bulge DNA 12/14mer: CGATATTAAGAGCC
bulge DNA 12/14mer: GGCTCAATATCG

Data sets:
Data typeCount
1H chemical shifts455
13C chemical shifts46
15N chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HMG-D protein1
2bulge DNA 12/14mer strand 12
3bulge DNA 12/14mer strand 23

Entities:

Entity 1, HMG-D protein 112 residues - Formula weight is not available

1   METSERASPLYSPROLYSARGPROLEUSER
2   ALATYRMETLEUTRPLEUASNSERALAARG
3   GLUSERILELYSARGGLUASNPROGLYILE
4   LYSVALTHRGLUVALALALYSARGGLYGLY
5   GLULEUTRPARGALAMETLYSASPLYSSER
6   GLUTRPGLUALALYSALAALALYSALALYS
7   ASPASPTYRASPARGALAVALLYSGLUPHE
8   GLUALAASNGLYGLYSERSERALAALAASN
9   GLYGLYGLYALALYSLYSARGALALYSPRO
10   ALALYSLYSVALALALYSLYSSERLYSLYS
11   GLUGLUSERASPGLUASPASPASPASPGLU
12   SERGLU

Entity 2, bulge DNA 12/14mer strand 1 14 residues - Formula weight is not available

1   DCDGDADTDADTDTDADADG
2   DADGDCDC

Entity 3, bulge DNA 12/14mer strand 2 12 residues - Formula weight is not available

1   DGDGDCDTDCDADADTDADT
2   DCDG

Samples:

sample_1: High Mobility Protein of Drosophila, [U-95% 15N; U-95% 13C], 1 mM; bulge DNA 12/14mer 1 mM; bulge DNA 12/14mer 1 mM

cond_1: pH: 6.0; temperature: 303 K; ionic strength: 0.005 M

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablecond_1
2D 1H-15N HSQCsample_1not availablecond_1
3D HNCAsample_1not availablecond_1
3D CBCA(CO)NHsample_1not availablecond_1

Software:

Xwin-NMR -

Felix -

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 4732
PDB
EMBL CAA50468 CAL26283 CAL26284 CAL26285 CAL26286
GB AAA28609 AAF46759 AAM48362 AAM71011 AAR09820
REF NP_001163244 NP_001286696 NP_726109 NP_726110 XP_001975105
SP Q05783