BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4358

Title: NMR studies of the Pbx1 TALE Homeodomain Protein free in Solution and bound to DNA : Proposal for a Mechanism of HoxB1-Pbx1-DNA Complex Assembly

Authors: Jabet, Carole; Gitti, Rossitza; Summers, Michael; Wolberger, Cynthia

Citation: Jabet, Carole; Gitti, Rossitza; Summers, Michael; Wolberger, Cynthia. "NMR studies of the Pbx1 TALE Homeodomain Protein free in Solution and bound to DNA : Proposal for a Mechanism of HoxB1-Pbx1-DNA Complex Assembly"  J. Mol. Biol. 291, 521-530 (1999).

Assembly members:
Pbx1, polymer, 87 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Pbx1: ARRKRRNFNKQATEILNEYF YSHLSNPYPSEEAKEELAKK CGITVSQVSNWFGNKRIRYK KNIGKFQEEANIYAAKTAVT ATNVSAH

Data sets:
Data typeCount
1H chemical shifts72
13C chemical shifts71
15N chemical shifts72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pbx1-HD-long1

Entities:

Entity 1, Pbx1-HD-long 87 residues - Formula weight is not available

1   ALAARGARGLYSARGARGASNPHEASNLYS
2   GLNALATHRGLUILELEUASNGLUTYRPHE
3   TYRSERHISLEUSERASNPROTYRPROSER
4   GLUGLUALALYSGLUGLULEUALALYSLYS
5   CYSGLYILETHRVALSERGLNVALSERASN
6   TRPPHEGLYASNLYSARGILEARGTYRLYS
7   LYSASNILEGLYLYSPHEGLNGLUGLUALA
8   ASNILETYRALAALALYSTHRALAVALTHR
9   ALATHRASNVALSERALAHIS

Samples:

Pbx1-HD+C_sample_1: Pbx1, [U-90% 15N], 1 mM

Pbx1-HD+C_sample_2: Pbx1, [U-95% 13C; U-90% 15N], 1 mM

sample_conditions_1: pH: 7 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available

Software:

NMRPipe - data processing

NMRView - peak assignments

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DMX 600 MHz

Related Database Links:

BMRB 4357 4359
PDB
DBJ BAA96135 BAA96136 BAB83538 BAF84104 BAG10741
GB AAA21832 AAA36484 AAA36764 AAA60031 AAB71191
PIR B33061
REF NP_001094151 NP_001128334 NP_001179697 NP_001191890 NP_001191892
SP P40424 P41778
TPG DAA32038