BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4247

Title: 1H Chemical Shift Assignments of the 8mer Chimeric Hybrid Duplex r(GCAGUGGC).r(RCCA)d(CTGC)

Authors: Szyperski, Thomas; Goette, Matthias; Billeter, Martin; Perola, Emanuele; Celai, Luciano; Heumann, Hermann; Wuthrich, Kurt

Citation: Szyperski, Thomas; Goette, Matthias; Billeter, Martin; Perola, Emanuele; Celai, Luciano; Heumann, Hermann; Wuthrich, Kurt. "NMR Structure of the Chimeric Hybrid Duplex r(gcaguggc). r(gcca)d(CTGC) Comprising the tRNA-DNA Junction Formed During Initiation of HIV-1 Reverse Trancription"  J. Biomol. NMR 13, 343-355 (1999).

Assembly members:
CHD_strand_A, polymer, 8 residues, Formula weight is not available
CHD_strand_B_RNA, polymer, 4 residues, Formula weight is not available
CHD_strand_B_DNA, polymer, 4 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eucaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: Chemical synthesis

Entity Sequences (FASTA):
CHD_strand_A: GCAGUGGC
CHD_strand_B_RNA: GCCA
CHD_strand_B_DNA: CTGC

Data typeCount
1H chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CHDA1
2CHDB RNA2
3CHDB DNA3

Entities:

Entity 1, CHDA 8 residues - Formula weight is not available

1   GCAGUGGC

Entity 2, CHDB RNA 4 residues - Formula weight is not available

1   GCCA

Entity 3, CHDB DNA 4 residues - Formula weight is not available

1   DCDTDGDC

Samples:

sample_one: 8mer chimeric hybrid RNA/RNA-DNA duplex with tRNA-DNA junction 1.5 mM; KPO4 20 mM; NaCL 100 mM; D2O 10%; H2O 90%

sample_conditions_one: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz
  • BRUKER AMX 600 MHz
  • VARIAN UNITY_PLUS 750 MHz

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