BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 34186

Title: Quadruplex with flipped tetrad formed by the c-myc promoter sequence   PubMed: 29756658

Authors: Karg, B.; Weisz, K.

Citation: Karg, B.; Weisz, K.. "Loop Length Affects Syn-Anti Conformational Rearrangements in Parallel G-Quadruplexes."  Chemistry ., .-. (2018).

Assembly members:
entity_1, polymer, 22 residues, 7324.094 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: TGAXGGTXGGTAXGGTXGGT AA

Data sets:
Data typeCount
1H chemical shifts158

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 22 residues - 7324.094 Da.

1   DTDGDABGMDGDGDTBGMDGDG
2   DTDABGMDGDGDTBGMDGDGDT
3   DADA

Samples:

sample_1: entity_1 mM; potassium phosphate 10 mM

sample_2: entity_1 mM; potassium phosphate 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7 pH*; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement, structure calculation

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

CcpNMR, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

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