BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 34168

Title: G-quadruplex structure of DNA oligonucleotide containing GGGGCC repeats linked to ALS and FTD

Authors: Brcic, J.; Plavec, J.

Citation: Brcic, J.; Plavec, J.. "G-quadruplex structure of DNA oligonucleotide with GGGGCC repeats, linked to ALS and FTD"  . ., .-..

Assembly members:
entity_1, polymer, 22 residues, 7036.323 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: GGGGCCGGGGCCGGGGCCGG XG

Data sets:
Data typeCount
1H chemical shifts176

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 22 residues - 7036.323 Da.

1   DGDGDGDGDCDCDGDGDGDG
2   DCDCDGDGDGDGDCDCDGDG
3   BGMDG

Samples:

sample_1: entity_1 1.0 mM; potassium chloride 30 mM; potassium phosphate 20 mM

sample_2: entity_1 0.4 mM; potassium chloride 30 mM; potassium phosphate 20 mM

sample_3: entity_1, one guanine in the sequence labeled 5% 15N and 13 C, 0.3 mM; potassium chloride 30 mM; potassium phosphate 20 mM

sample_conditions_1: ionic strength: 53 mM; pH: 5.8; pressure: 1 bar; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
1D 15N-edited HSQCsample_3isotropicsample_conditions_1
1D 13C-edited HSQCsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

AMBER v14, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement, structure calculation

SPARKY, Goddard - chemical shift assignment, peak picking

VNMR, Varian - processing

NMR spectrometers:

  • Agilent-Varian VNMRS 600 MHz
  • Agilent-Varian VNMRS 800 MHz

Related Database Links: