BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 30055

Title: DIY G-Quadruplexes: Solution structure of d(GGTTTGGTTTTGGTTTGG) in sodium

Authors: Dvorkin, S.; Webba da Silva, M.

Citation: Dvorkin, S.; Webba da Silva, M.. "DIY G-Quadruplexes: Solution structure of d(GGTTTGGTTTTGGTTTGG) in sodium"  . ., .-..

Assembly members:
DNA (5'-D(*GP*GP*TP*TP*TP*GP*GP*TP*TP*TP*TP*GP*GP*TP*TP*TP*GP*G)-3'), polymer, 18 residues, 5630.618 Da.

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA (5'-D(*GP*GP*TP*TP*TP*GP*GP*TP*TP*TP*TP*GP*GP*TP*TP*TP*GP*G)-3'): GGTTTGGTTTTGGTTTGG

Data sets:
Data typeCount
1H chemical shifts193

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 18 residues - 5630.618 Da.

1   DGDGDTDTDTDGDGDTDTDT
2   DTDGDGDTDTDTDGDG

Samples:

sample_1: DNA, none, 2 ± 0.5 mM; NaPi 100 mM; H2O 90%; D2O 10%

sample_2: DNA, none, 2 ± 0.5 mM; NaPi 100 mM; D2O 100%

sample_conditions_1: ionic strength: 100 mM NaPi mM; pH: 6.8; pressure: 1 atm; temperature: 278.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

Felix, Accelrys Software Inc. - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

NMR spectrometers:

  • Varian VXRS 500 MHz

Related Database Links: