BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25915

Title: Photoswitchable G-quadruplex   PubMed: 26805928

Authors: Thevarpadam, Julie; Bessi, Irene; Binas, Oliver; Goncalves, Diana; Slavov, Chavdar; Jonker, Hendrik; Richter, Christian; Wachtveitl, Josef; Schwalbe, Harald; Heckel, Alexander

Citation: Thevarpadam, Julie; Bessi, Irene; Binas, Oliver; Goncalves, Diana; Slavov, Chavdar; Jonker, Hendrik; Richter, Christian; Wachtveitl, Josef; Schwalbe, Harald; Heckel, Alexander. "Photoresponsive formation of an intermolecular minimal G-quadruplex motif"  Angew. Chem. Int. Ed. 55, 2738-2742 (2016).

Assembly members:
DNA_(5'-D(*GP*GP*(AZW)P*GP*G)-3'), polymer, 5 residues, 1252.861 Da.
entity_K, non-polymer, 39.098 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: DNA solid-phase synthesis   Host organism: not applicable

Entity Sequences (FASTA):
DNA_(5'-D(*GP*GP*(AZW)P*GP*G)-3'): GGXGG

Data sets:
Data typeCount
13C chemical shifts34
1H chemical shifts48

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA (5'-D(*GP*GP*(AZW)P*GP*G)-3')_11
2DNA (5'-D(*GP*GP*(AZW)P*GP*G)-3')_21
3DNA (5'-D(*GP*GP*(AZW)P*GP*G)-3')_31
4DNA (5'-D(*GP*GP*(AZW)P*GP*G)-3')_41
5POTASSIUM ION_12
6POTASSIUM ION_22
7POTASSIUM ION_32

Entities:

Entity 1, DNA (5'-D(*GP*GP*(AZW)P*GP*G)-3')_1 5 residues - 1252.861 Da.

1   DGDGAZWDGDG

Entity 2, POTASSIUM ION_1 - K - 39.098 Da.

1   K

Samples:

sample_H2O: DNA (5'-D(*GP*GP*(AZW)P*GP*G)-3') 550 uM; Tris-HCl 25 mM; KCl 50 mM

sample_D2O: DNA (5'-D(*GP*GP*(AZW)P*GP*G)-3') 550 uM; Tris-HCl 25 mM; KCl 50 mM

sample_conditions: ionic strength: 71 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_D2Oisotropicsample_conditions
2D 1H-13C HMBCsample_H2Oisotropicsample_conditions
2D 1H-31P TOCSYsample_D2Oisotropicsample_conditions
2D 1H-1H P.E. COSYsample_D2Oisotropicsample_conditions
2D 1H-1H NOESYsample_H2Oisotropicsample_conditions
2D 1H-1H NOESYsample_D2Oisotropicsample_conditions

Software:

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

ARIA v1.2, Linge, O'Donoghue and Nilges - refinement, structure solution

TOPSPIN v3.2, Bruker Biospin - collection, processing

SPARKY v3.114, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links: