BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25407

Title: Structure of the DNA complex of the C-Terminal domain of MvaT   PubMed: 26068099

Authors: Ding, Pengfei; Xia, Bin

Citation: Ding, Pengfei; McFarland, Kirsty; Jin, Shujuan; Tong, Grace; Duan, Bo; Yang, Ally; Hughes, Timothy; Liu, Jun; Dove, Simon; Navarre, William; Xia, Bin. "A Novel AT-Rich DNA Recognition Mechanism for Bacterial Xenogeneic Silencer MvaT"  Plos Pathog. 11, e1004967-e1004967 (2015).

Assembly members:
MvaT, polymer, 55 residues, 5352.173 Da.
DNA_(5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3'), polymer, 12 residues, 3662.434 Da.

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MvaT: MKRARKVKQYKNPHTGEVIE TKGGNHKTLKEWKAKWGPEA VESWATLLGHHHHHH
DNA_(5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3'): CGCATATATGCG

Data sets:
Data typeCount
13C chemical shifts163
15N chemical shifts51
1H chemical shifts536

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MvaT1
2DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3')_12
3DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3')_22

Entities:

Entity 1, MvaT 55 residues - 5352.173 Da.

1   METLYSARGALAARGLYSVALLYSGLNTYR
2   LYSASNPROHISTHRGLYGLUVALILEGLU
3   THRLYSGLYGLYASNHISLYSTHRLEULYS
4   GLUTRPLYSALALYSTRPGLYPROGLUALA
5   VALGLUSERTRPALATHRLEULEUGLYHIS
6   HISHISHISHISHIS

Entity 2, DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3')_1 12 residues - 3662.434 Da.

1   DCDGDCDADTDADTDADTDG
2   DCDG

Samples:

sample_1: MvaT, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 50 mM; DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3') 1 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 25405
PDB
DBJ BAK88160 BAP21250 BAP52811 BAQ41953 BAR69621
EMBL CAW29447 CCQ87551 CDH72901 CDH79198 CDI92830
GB AAG07703 AAP33788 ABJ13585 ABR84836 AEO76886
REF NP_253005 WP_003093888 WP_003125172 WP_012077126 WP_015478773