BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19886

Title: MINOR GROOVE RECOGNITION OF DNA BY THIAZOTROPSIN ANALOGUES   PubMed: 25045155

Authors: Alniss, H.; Salvia, M.; Sadikov, M.; Golovchenko, I.; Anthony, N.; Khalaf, A.; Mackay, S.; Suckling, C.; Parkinson, J.

Citation: Alniss, H.; Salvia, M.; Sadikov, M.; Golovchenko, I.; Anthony, N.; Khalaf, A.; Mackay, S.; Suckling, C.; Parkinson, J.. "Recognition of the DNA minor groove by thiazotropsin analogues"  Chembiochem 15, 1978-1990 (2014).

Assembly members:
5'-D(*CP*GP*AP*CP*GP*CP*GP*TP*CP*G)-3', polymer, 10 residues, 3046.017 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):
5'-D(*CP*GP*AP*CP*GP*CP*GP*TP*CP*G)-3': CGACGCGTCG

Data sets:
Data typeCount
1H chemical shifts98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
15'-D(*CP*GP*AP*CP*GP*CP*GP*TP*CP*G)-3'1

Entities:

Entity 1, 5'-D(*CP*GP*AP*CP*GP*CP*GP*TP*CP*G)-3' 10 residues - 3046.017 Da.

1   DCDGDADCDGDCDGDTDCDG

Samples:

sample_1: 5'-D(*CP*GP*AP*CP*GP*CP*GP*TP*CP*G)-3' 2 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1

Software:

AMBER, Bruker Biospin, Goddard - chemical shift assignment, collection, refinement

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links: