BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18524

Title: Solution structure of a parallel-stranded oligoisoguanine DNA pentaplex formed by d(T(iG)4T) in the presence of Cs ions   PubMed: 22767484

Authors: Kang, Mijeong; Heuberger, Ben; Chaput, John; Switzer, Christopher; Feigon, Juli

Citation: Kang, Mijeong; Heuberger, Ben; Chaput, John; Switzer, Christopher; Feigon, Juli. "Solution structure of a parallel-stranded oligoisoguanine DNA pentaplex formed by d(T(iG)4 T) in the presence of Cs+ ions."  Angew. Chem. Int. Ed. Engl. 51, 7952-7955 (2012).

Assembly members:
DNA_OUH, polymer, 6 residues, 1880.268 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA_OUH: TXXXXT

Data sets:
Data typeCount
13C chemical shifts130
1H chemical shifts325

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA_11
2DNA_21
3DNA_31
4DNA_41
5DNA_51

Entities:

Entity 1, DNA_1 6 residues - 1880.268 Da.

1   DTIGUIGUIGUIGUDT

Samples:

sample_1: DNA 0.7 mM; H2O 90%; D2O 10%

sample_2: DNA 0.7 mM; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 6.3; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

xwinnmr, Bruker Biospin - processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker Avance 800 MHz

Related Database Links: