BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17228

Title: C-terminal zinc knuckle of the HIVNCp7   PubMed: 22303928

Authors: Quintal, Susana; Viegas, Aldino; Cabrita, Eurico; Farrell, Nicholas; Erhardt, Stefan

Citation: Quintal, Susana; Viegas, Aldino; Erhardt, Stefan; Cabrita, Eurico; Farrell, Nicholas. "Platinated DNA Affects Zinc Finger Conformation. Interaction of a Platinated Single-Stranded Oligonucleotide and the C-Terminal Zinc Finger of Nucleocapsid Protein HIVNCp7."  Biochemistry 51, 1752-1761 (2012).

Assembly members:
C-terminal_zinc_knuckle_of_the_HIVNCp7, polymer, 19 residues, 2230.615 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: HIV   Taxonomy ID: 12721   Superkingdom: viruses   Kingdom: not available   Genus/species: Lentivirus HIV

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):
C-terminal_zinc_knuckle_of_the_HIVNCp7: KGCWKCGKEGHQMKDCTER

Data sets:
Data typeCount
1H chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1
2ZINC_ION_12
3ZINC_ION_22
4ZINC_ION_32
5ZINC_ION_42

Entities:

Entity 1, entity 19 residues - 2230.615 Da.

1   LYSGLYCYSTRPLYSCYSGLYLYSGLUGLY
2   HISGLNMETLYSASPCYSTHRGLUARG

Entity 2, ZINC_ION_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: C-terminal zinc knuckle of the HIVNCp7 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 6 mM; pH: 5.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY v3.114, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 1656 17216 17229 18980 2217 25532
PDB
DBJ BAA12988 BAA12996 BAA84661 BAA84662 BAA84669
EMBL CAA28011 CAA42971 CAA42973 CAA73647 CAA77483
GB AAA43911 AAA44201 AAA44313 AAA44314 AAA44317
PIR FOVWLV
PRF 1102247B 1103299C
REF NP_057849 NP_057850 NP_579881
SP O89939 O89940 P03347 P03348 P03366