BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7149

Title: Characterization of the Molten Globule of Human Serum Retinol-Binding Protein using NMR Spectroscopy   PubMed: 16878982

Authors: Greene, Lesley; Wijesinha-Bettoni, Ramani; Redfield, Christina

Citation: Greene, Lesley; Wijesinha-Bettoni, Ramani; Redfield, Christina. "Characterization of the molten globule of human serum retinol-binding protein using NMR spectroscopy"  Biochemistry 45, 9475-9484 (2006).

Assembly members:
human serum retinol-binding protein, polymer, 182 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
human serum retinol-binding protein: ERDCRVSSFRVKENFDKARF SGTWYAMAKKDPEGLFLQDN IVAEFSVDETGQMSATAKGR VRLLNNWDVCADMVGTFTDT EDPAKFKMKYWGVASFLQKG NDDHWIVDTDYDTYAVQYSC RLLNLDGTCADSYSFVFSRD PNGLPPEAQKIVRQRQEELC LARQYRLIVHNGYCDGRSER NL

Data sets:
Data typeCount
15N chemical shifts174
1H chemical shifts174

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RBP1

Entities:

Entity 1, RBP 182 residues - Formula weight is not available

1   GLUARGASPCYSARGVALSERSERPHEARG
2   VALLYSGLUASNPHEASPLYSALAARGPHE
3   SERGLYTHRTRPTYRALAMETALALYSLYS
4   ASPPROGLUGLYLEUPHELEUGLNASPASN
5   ILEVALALAGLUPHESERVALASPGLUTHR
6   GLYGLNMETSERALATHRALALYSGLYARG
7   VALARGLEULEUASNASNTRPASPVALCYS
8   ALAASPMETVALGLYTHRPHETHRASPTHR
9   GLUASPPROALALYSPHELYSMETLYSTYR
10   TRPGLYVALALASERPHELEUGLNLYSGLY
11   ASNASPASPHISTRPILEVALASPTHRASP
12   TYRASPTHRTYRALAVALGLNTYRSERCYS
13   ARGLEULEUASNLEUASPGLYTHRCYSALA
14   ASPSERTYRSERPHEVALPHESERARGASP
15   PROASNGLYLEUPROPROGLUALAGLNLYS
16   ILEVALARGGLNARGGLNGLUGLULEUCYS
17   LEUALAARGGLNTYRARGLEUILEVALHIS
18   ASNGLYTYRCYSASPGLYARGSERGLUARG
19   ASNLEU

Samples:

sample_1: human serum retinol-binding protein, [U-15N; U-2H], 0.16 mM; sodium phosphate 5 mM; urea 8 M

conditions_1: pH: 2; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1

Software:

FELIX v2.3, Accelrys - processing

NMR spectrometers:

  • Home-built . 750 MHz

Related Database Links:

PDB
DBJ BAD16621 BAI46108
EMBL CAA24959 CAA26553
GB AAF69622 AAH20633 ABC69164 ABM82307 ABM85484
PRF 1401251A
REF NP_001038960 NP_006735 XP_002821040 XP_003255281 XP_003904062
SP P02753 P61641