BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6968

Title: 1H, 13C and 15N assignment of C', CA, CB, N, HN, HA and HB of intrinsically disordered alpha-synuclein   PubMed: 16551093

Authors: Bermel, Wolfgang; Bertini, Ivano; Felli, Isabella; Lee, Yong-Min; Luchinat, Claudio; Kozminski, Wiktor; Piai, Alessandro; Pierattelli, Roberta; Stanek, Jan; Zawadzka-Kazimierczuk, Anna

Citation: Bermel, Wolfgang; Bertini, Ivano; Felli, Isabella; Lee, Yong-Min; Luchinat, Claudio; Pierattelli, Roberta. "Protonless NMR experiments for sequence-specific assignment of backbone nuclei in unfolded proteins"  J. Am. Chem. Soc. 128, 3918-3919 (2006).

Assembly members:
intrinsically disordered alpha-synuclein, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
intrinsically disordered alpha-synuclein: MDVFMKGLSKAKEGVVAAAE KTKQGVAEAAGKTKEGVLYV GSKTKEGVVHGVATVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data sets:
Data typeCount
1H chemical shifts523
13C chemical shifts401
15N chemical shifts139

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1intrinsically disordered alpha-synuclein1

Entities:

Entity 1, intrinsically disordered alpha-synuclein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: intrinsically disordered alpha-synuclein, [U-13C; U-15N], 1 mM; KPi 20 mM; EDTA 0.5 mM; NaCl 200 mM; H2O 90%; D2O 10%

conditions_1: pH: 6.5; temperature: 285.5 K

Experiments:

NameSampleSample stateSample conditions
COCON-IPAPsample_1not availableconditions_1
CBCACON-IPAPsample_1not availableconditions_1
HCBCACON-IPAPsample_1not availableconditions_1
HCBCANCO-IPAPsample_1not availableconditions_1
HNCACON-IPAPsample_1not availableconditions_1
HNCANCO-IPAPsample_1not availableconditions_1

Software:

TOPSPIN, Bruker -

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 16300 16302 16342 16543 16546 16547 16548 16904 16939 17214 17498 17648 17649 17654 17665 17910 18207 18208 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 5744
PDB
DBJ BAB29375 BAF82858 BAG73790
EMBL CAG33339 CAG46454
GB AAA16117 AAC02114 AAG30302 AAH13293 AAI08276
REF NP_000336 NP_001009158 NP_001032222 NP_001129014 NP_001139526
SP P37840 P61139 P61140 P61142 P61143