BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6962

Title: Resonance Assignments of 10:0-ACP   PubMed: 16618110

Authors: Zornetzer, Gregory; Fox, Brian; Markley, John

Citation: Zornetzer, Gregory; Fox, Brian; Markley, John. "Solution Structures of Spinach Acyl Carrier Protein with Decanoate and Stearate"  Biochemistry 45, 5217-5227 (2006).

Assembly members:
Acyl Carrier Protein, polymer, 82 residues, Formula weight is not available
PNS, non-polymer, 358.348 Da.
DKA, non-polymer, 172.265 Da.

Natural source:   Common Name: Spinach   Taxonomy ID: 3562   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Spinacia oleracea

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Acyl Carrier Protein: AKKETIDKVSDIVKEKLALG ADVVVTADSEFSKLGADSLD TVEIVMNLEEEFGINVDEDK AQDISTIQQAADVIEGLLEK KA

Data sets:
Data typeCount
13C chemical shifts343
15N chemical shifts86
1H chemical shifts612

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1acyl carrier protein1
2Phosphopantetheine2
3Decanoate3

Entities:

Entity 1, acyl carrier protein 82 residues - Formula weight is not available

1   ALALYSLYSGLUTHRILEASPLYSVALSER
2   ASPILEVALLYSGLULYSLEUALALEUGLY
3   ALAASPVALVALVALTHRALAASPSERGLU
4   PHESERLYSLEUGLYALAASPSERLEUASP
5   THRVALGLUILEVALMETASNLEUGLUGLU
6   GLUPHEGLYILEASNVALASPGLUASPLYS
7   ALAGLNASPILESERTHRILEGLNGLNALA
8   ALAASPVALILEGLUGLYLEULEUGLULYS
9   LYSALA

Entity 2, Phosphopantetheine - C11 H23 N2 O7 P S - 358.348 Da.

1   PNS

Entity 3, Decanoate - C10 H20 O2 - 172.265 Da.

1   DKA

Samples:

15N_10-0-ACP: Acyl Carrier Protein, [U-15N], 2 ± 0.1 mM; MES pH 6.1 10 ± 1 mM; NaCl 100 ± 3 mM

13C-15N_10-0-ACP: Acyl Carrier Protein, [U-15N; U-13C], 2 ± 0.1 mM; MES pH 6.1 10 ± 1 mM; NaCl 100 ± 3 mM

conditions_1: ionic strength: 100 mM; pH: 6.1; temperature: 287 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availableconditions_1
TOCSY-15N HSQCnot availablenot availableconditions_1
NOESY-15N HSQCnot availablenot availableconditions_1
HNCO13C-15N_10-0-ACPnot availableconditions_1
HNCACB13C-15N_10-0-ACPnot availableconditions_1
C(CO)NH TOCSYnot availablenot availableconditions_1
H(CCO)NH TOCSYnot availablenot availableconditions_1
H(C)CH TOCSYnot availablenot availableconditions_1
NOESY-13C HSQCnot availablenot availableconditions_1
NOESY-aromatic 13C HSQCnot availablenot availableconditions_1
f1-filtered 1H NOESYnot availablenot availableconditions_1
f1-filtered 1H TOCSYnot availablenot availableconditions_1
f1-filtered NOESY-15N HSQCnot availablenot availableconditions_1
f1-filtered NOESY-13C HSQCnot availablenot availableconditions_1

Software:

xwinnmr v2.6, Bruker - Data acquisition

VNMR v6.1, Varian, Inc. - data acquisition

NMRPipe, Bax Group: NIH - data processing, automated peak picking, angular restraint generation (TALOS)

SPARKY v3.111, UCSF - manual peak picking, assignment

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links: