BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6939

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Ubiquitin Specific Protease 7   PubMed: 15808506

Authors: Sheng, Yi; Saridakis, Vivian; Liao, Jack; Frappier, Lori; Arrowsmith, Cheryl

Citation: Saridakis, V.; Sheng, Yi; Sarkari, F.; Holowaty, M.N.; Shire, F.; Nguyen, T.; Zhang, R.G.; Liao, F.; Lee, W.; Edwards, A.M.; Arrowsmith, Cheryl; Frappier, L.. "'1H,15N and 13C Assigned Chemical Shifts for USP7 in a complex with an EBNA1 peptide"  Mol. Cell 18, 25-36 (2005).

Assembly members:
USP7, polymer, 144 residues, Formula weight is not available
EBNA1, polymer, . residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapien

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
USP7: DTSWRSEATFQFTVERFSRL SESVLSPPCFVRNLPWKIMV MPRFYPDRPHQKSVGFFLQC NAESDSTSWSCHAQAVLKII NYRDDEKSFSRRISHLFFHK ENDWGFSNFMAWSEVTDPEK GFIDDDKVTFEVFVQADAPH GVAW

Data sets:
Data typeCount
13C chemical shifts252
15N chemical shifts123
1H chemical shifts123

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1USP71
2EBNA12

Entities:

Entity 1, USP7 144 residues - Formula weight is not available

1   ASPTHRSERTRPARGSERGLUALATHRPHE
2   GLNPHETHRVALGLUARGPHESERARGLEU
3   SERGLUSERVALLEUSERPROPROCYSPHE
4   VALARGASNLEUPROTRPLYSILEMETVAL
5   METPROARGPHETYRPROASPARGPROHIS
6   GLNLYSSERVALGLYPHEPHELEUGLNCYS
7   ASNALAGLUSERASPSERTHRSERTRPSER
8   CYSHISALAGLNALAVALLEULYSILEILE
9   ASNTYRARGASPASPGLULYSSERPHESER
10   ARGARGILESERHISLEUPHEPHEHISLYS
11   GLUASNASPTRPGLYPHESERASNPHEMET
12   ALATRPSERGLUVALTHRASPPROGLULYS
13   GLYPHEILEASPASPASPLYSVALTHRPHE
14   GLUVALPHEVALGLNALAASPALAPROHIS
15   GLYVALALATRP

Entity 2, EBNA1 - Formula weight is not available

Samples:

sample_1: USP7, [U-95% 13C; U-90% 15N], 0.7 mM; EBNA1 3 mM; sodium phosphate 20 mM; sodium chloride 250 mM

conditions_1: pH: 7.4; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
H-15N HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CCCTOCSYNNHsample_1not availableconditions_1
HSQCsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAE22671 BAH13721 BAH13801 BAH13828 BAH13841
EMBL CAA96580
GB AAI66012 AAI66690 AAQ12339 AAQ82908 AAT68666
REF NP_001003918 NP_001019961 NP_001129152 NP_001273386 NP_001273387
SP Q4VSI4 Q6A4J8 Q93009
TPG DAA15729