BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5877

Title: Solid State NMR Structure of the Major Coat Protein in Bacteriophage Pf1   PubMed: 15288792

Authors: Thiriot, D.; Opella, S.

Citation: Thiriot, D.; Nevzorov, A.; Zagyanskiy, L.; Wu, C.; Opella, S.. "Structure of the coat protein in Pf1 bacteriophage determined by solid-state NMR spectroscopy."  J. Mol. Biol. 341, 869-879 (2004).

Assembly members:
Pf1 phage major coat protein, polymer, 46 residues, Formula weight is not available

Natural source:   Common Name: Pseudomonas phage Pf1   Taxonomy ID: 10871   Superkingdom: Viruses   Kingdom: not available   Genus/species: Inovirus Pseudomonas phage Pf1

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
Pf1 phage major coat protein: GVIDTSAVESAITDGQGDMK AIGGYIVGALVILAVAGLIY SMLRKA

Data sets:
Data typeCount
15N chemical shifts43
coupling constants43

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pf1 major coat protein1

Entities:

Entity 1, Pf1 major coat protein 46 residues - Formula weight is not available

1   GLYVALILEASPTHRSERALAVALGLUSER
2   ALAILETHRASPGLYGLNGLYASPMETLYS
3   ALAILEGLYGLYTYRILEVALGLYALALEU
4   VALILELEUALAVALALAGLYLEUILETYR
5   SERMETLEUARGLYSALA

Samples:

sample_1: Pf1 phage major coat protein, [U-15N], 50 mg/mL; sodium borate buffer 5 mM; H2O M

sample_cond_1: pH: 8 na; temperature: 303 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
PISEMAnot availablenot availablenot available
Magic Sammynot availablenot availablenot available

Software:

FELIX v2000.1 - processing

XWINNMR, MacNMR, NTNMR - collection

MATLAB script v6.5 - structure solution

MATLAB scripts v6.5 - refinement

SCWRL v2.95 - Blue Horizon modified - refinement

SCWRL v2.95 - refinement

NMR spectrometers:

  • Bruker AVANCE 750 MHz
  • Homebuilt Apollo 550 MHz

Related Database Links:

BMRB 15138
PDB
EMBL CAA36331 CCQ85809 CDH73426 CDO83853
GB AAG04112 AAT50608 ACS36651 AGV62109 AGV64452
PRF 754922A
REF NP_039603 NP_249414 NP_955532 WP_003125072 WP_019372527
SP P03621