BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5660

Title: Partial proton resonance assignements for the different conformers of ferricytochrome c produced in 30% acetonitrile and 70% water solution

Authors: Sivakolundu, Sivashankar; Mabrouk, Patricia

Citation: Sivakolundu, Sivashankar; Mabrouk, Patricia. "Insights into the Alkaline Transformation of Ferricyt c from 1H NMR Study in 30% Acetonitrile-Water"  Protein Sci. 10, 2291-2300 (2001).

Assembly members:
oxidized cytochrome c, polymer, 104 residues, 12384 Da.
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: Horse   Taxonomy ID: 9796   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Equus caballus

Experimental source:   Production method: vendor

Entity Sequences (FASTA):
oxidized cytochrome c: GDVEKGKKIFVQKCAQCHTV EKGGKHKTGPNLHGLFGRKT GQAPGFTYTDANKNKGITWK EETLMEYLENPKKYIPGTKM IFAGIKKKTEREDLIAYLKK ATNE

Data typeCount
1H chemical shifts39

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c1
2HEME C2

Entities:

Entity 1, cytochrome c 104 residues - 12384 Da.

1   GLYASPVALGLULYSGLYLYSLYSILEPHE
2   VALGLNLYSCYSALAGLNCYSHISTHRVAL
3   GLULYSGLYGLYLYSHISLYSTHRGLYPRO
4   ASNLEUHISGLYLEUPHEGLYARGLYSTHR
5   GLYGLNALAPROGLYPHETHRTYRTHRASP
6   ALAASNLYSASNLYSGLYILETHRTRPLYS
7   GLUGLUTHRLEUMETGLUTYRLEUGLUASN
8   PROLYSLYSTYRILEPROGLYTHRLYSMET
9   ILEPHEALAGLYILELYSLYSLYSTHRGLU
10   ARGGLUASPLEUILEALATYRLEULYSLYS
11   ALATHRASNGLU

Entity 2, HEME C - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: oxidized cytochrome c 2 mM; PROTOPORPHYRIN IX CONTAINING FE 2 mM; acetonitrile 30%; H2O 70%

Ex-cond_1: pH*: 7.2; temperature: 293 K

Ex-cond_2: pH*: 7.2; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H NOESYsample_1not availablenot available

Software:

FELIX v2000 - spectral processing, spectral analysis

NMR spectrometers:

  • Varian Mercury 300 MHz
  • home made . 500 MHz
  • home made . 600 MHz

Related Database Links:

SWISS-PROT P68097 P68096 P00004
REF XP_001498872
PRF 610169A
GenBank AAB33495
PDB
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