BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5351

Title: Backbone HN, N, Ca, Cb and CO assignments of unfolded ACBP   PubMed: 12441112

Authors: Poulsen, Flemming

Citation: Teilum, Kaare; Kragelund, Birthe; Poulsen, Flemming. "Transient Structure Formation in Unfolded Acyl-coenzyme A-binding Protein Observed by Site-directed Spin Labelling"  J. Mol. Biol. 324, 349-357 (2002).

Assembly members:
acyl-coenzyme A binding protein, polymer, 86 residues, Formula weight is not available
MTN, non-polymer, 264.385 Da.

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
acyl-coenzyme A binding protein: SQAEFDKAAEEVKHLKTKPA DEEMLFIYSHYKQATVGDIN TERPGMLDFKGKAKWDAWNE LKGTSKEDAMKAYIDKVEEL KKKYGC

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts223
15N chemical shifts80
1H chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ACBP1
2MTN2

Entities:

Entity 1, ACBP 86 residues - Formula weight is not available

1   SERGLNALAGLUPHEASPLYSALAALAGLU
2   GLUVALLYSHISLEULYSTHRLYSPROALA
3   ASPGLUGLUMETLEUPHEILETYRSERHIS
4   TYRLYSGLNALATHRVALGLYASPILEASN
5   THRGLUARGPROGLYMETLEUASPPHELYS
6   GLYLYSALALYSTRPASPALATRPASNGLU
7   LEULYSGLYTHRSERLYSGLUASPALAMET
8   LYSALATYRILEASPLYSVALGLUGLULEU
9   LYSLYSLYSTYRGLYCYS

Entity 2, MTN - C10 H18 N O3 S2 - 264.385 Da.

1   MTN

Samples:

Sample_1: acyl-coenzyme A binding protein, [U-13C; U-15N], 1.5 mM; guanidinium chloride 1.9 M

Experimental_Conditions: pH: 5.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCSample_1not availableExperimental_Conditions
HNCOSample_1not availableExperimental_Conditions
HN(CA)COSample_1not availableExperimental_Conditions
HN(CO)CASample_1not availableExperimental_Conditions
HNCASample_1not availableExperimental_Conditions
HNCACBSample_1not availableExperimental_Conditions
CBCA(CO)NHSample_1not availableExperimental_Conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 16419 2049 2050
PDB
EMBL CAA44618
GB AAA30495 AAI14182 AIM41257 ELR45212
REF NP_001106792 XP_004004799 XP_005896385 XP_005910864 XP_006040065
SP P07107
TPG DAA32604