BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5076

Title: Resonance Assignment of the unfolded states of Cold Shock Domain of the human YB-1 protein   PubMed: 9835056

Authors: Kloks, Cathelijne; Hoffmann, Astrid; Omichinski, James; Vuister, Geerten; Hilbers, Cornelis; Grzesiek, Stephan

Citation: Kloks, Cathelijne; Hoffmann, Astrid; Omichinski, James; Vuister, Geerten; Hilbers, Cornelis; Grzesiek, Stephan. "Resonance Assignment and Secondary Structure of the Cold Shock Domain of the Human YB-1 Protein"  J. Biomol. NMR 12, 463-464 (1998).

Assembly members:
Cold Shock domain, polymer, 79 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Cold Shock domain: MKKVIATKVLGTVKWFNVRN GYGFINRNDTKEDVFVHQTA IKKNNPRKYLRSVGDGETVE FDVVEGEKGAEAANVTGPG

Data typeCount
1H chemical shifts121
13C chemical shifts60
15N chemical shifts42
coupling constants17
T1 relaxation values63

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cold Shock domain1

Entities:

Entity 1, Cold Shock domain 79 residues - Formula weight is not available

1   METLYSLYSVALILEALATHRLYSVALLEU
2   GLYTHRVALLYSTRPPHEASNVALARGASN
3   GLYTYRGLYPHEILEASNARGASNASPTHR
4   LYSGLUASPVALPHEVALHISGLNTHRALA
5   ILELYSLYSASNASNPROARGLYSTYRLEU
6   ARGSERVALGLYASPGLYGLUTHRVALGLU
7   PHEASPVALVALGLUGLYGLULYSGLYALA
8   GLUALAALAASNVALTHRGLYPROGLY

Samples:

sample1: Cold Shock domain, [U-15N], 1.5 mM; H2O 90%; D2O 10%

sample2: Cold Shock domain, [U-15N; U-13C], 1.5 mM; H2O 90%; D2O 10%

sample3: Cold Shock domain, [U-15N; U-13C], 1.5 mM; D2O 100%

sample_conditions_one: pH: 6.7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions_one

Software:

nmrPipe -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Varian Inova 750 MHz
  • Varian Inova 500 MHz

Related Database Links:

BMRB 17225 4147
PDB
DBJ BAA02569 BAA03376 BAA05380 BAA19849 BAC45236
EMBL CAA40847 CAA42778 CAA51261 CAA64631 CAB46826
GB AAA02573 AAA20871 AAA21677 AAA30497 AAA35749
PRF 2210343A
REF NP_001003127 NP_001016677 NP_001076254 NP_001079367 NP_001080330
SP P16989 P21573 P62960 P62961 P67808
TPG DAA29365 DAA30957