BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4922

Title: Backbone 1H, 13C and 15N chemical shift assignments for unfolded apoplastocyanin   PubMed: 11316886

Authors: Bai, Yawen; Chung, John; Dyson, Jane; Wright, Peter

Citation: Bai, Yawen; Chung, John; Dyson, H. Jane; Wright, Peter. "Structural and Dynamic Characterization of an Unfolded State of Poplar Apo-plastocyanin Formed under Non-denaturing Conditions"  Protein Sci. 10, 1056-1066 (2001).

Assembly members:
unfolded apo-plastocyanin, polymer, 99 residues, Formula weight is not available

Natural source:   Common Name: poplar   Taxonomy ID: 3691   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Populus nigra

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
unfolded apo-plastocyanin: IDVLLGADDGSLAFVPSEFS ISPGEKIVFKNNAGFPHNIV FDEDSIPSGVDASKISMSEE DLLNAKGETFEVALSNKGEY SFYCSPHQGAGMVGKVTVN

Data sets:
Data typeCount
1H chemical shifts304
13C chemical shifts270
15N chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unfolded apo-Pc1

Entities:

Entity 1, unfolded apo-Pc 99 residues - Formula weight is not available

1   ILEASPVALLEULEUGLYALAASPASPGLY
2   SERLEUALAPHEVALPROSERGLUPHESER
3   ILESERPROGLYGLULYSILEVALPHELYS
4   ASNASNALAGLYPHEPROHISASNILEVAL
5   PHEASPGLUASPSERILEPROSERGLYVAL
6   ASPALASERLYSILESERMETSERGLUGLU
7   ASPLEULEUASNALALYSGLYGLUTHRPHE
8   GLUVALALALEUSERASNLYSGLYGLUTYR
9   SERPHETYRCYSSERPROHISGLNGLYALA
10   GLYMETVALGLYLYSVALTHRVALASN

Samples:

sample_1: unfolded apo-plastocyanin, [U-15N], 2.0 mM; KH2PO4 5 mM; EDTA 50 uM; beta-mercaptoethanol 5 mM

sample_2: unfolded apo-plastocyanin, [U-15N; U-13C], 1.5 mM; KH2PO4 5 mM; EDTA 50 uM; beta-mercaptoethanol 5 mM

sample_3: unfolded apo-plastocyanin, [U-15N; U-13C], 0.5 mM; KH2PO4 5 mM; EDTA 50 uM; beta-mercaptoethanol 5 mM

sample_4: unfolded apo-plastocyanin 0.1 mM; KH2PO4 5 mM; EDTA 50 uM; beta-mercaptoethanol 5 mM

Ex-cond_1: pH: 6.0; temperature: 308 K; ionic strength: 5 mM

Experiments:

NameSampleSample stateSample conditions
15N NOESY-HSQCnot availablenot availablenot available
C(CO)NH-TOCSYnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
TOCSY-HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
(HA)CO(CA)NH(particularly useful for unfolded proteins)not availablenot availablenot available

Software:

FELIX - Fourier transformation, analysis

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

BMRB 19247 4019
PDB
EMBL CAA90564
GB ABK96770 EEE94750
REF XP_002307754 XP_011003094
SP P00299