BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4676

Title: Assignment of 1H, 13C and 15N Resonances in Unfolded Apomyoglobin at pH 2.3   PubMed: 11297422

Authors: Yao, Jian; Chung, J.; Eliezer, D.; Wright, Peter; Dyson, Jane

Citation: Yao, Jian; Chung, J.; Eliezer, D.; Wright, Peter; Dyson, H.. "NMR Structural and Dynamic Characterization of the Acid-unfolded State of Apomyoglobin Provides Insights into the Early Events in Protein Folding"  Biochemistry 40, 3561-3571 (2001).

Assembly members:
unfolded myoglobin, polymer, 153 residues, Formula weight is not available

Natural source:   Common Name: sperm whale   Taxonomy ID: 9755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Physeter catodon

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
unfolded myoglobin: VLSEGEWQLVLHVWAKVEAD VAGHGQDILIRLFKSHPETL EKFDRFKHLKTEAEMKASED LKKHGVTVLTALGAILKKKG HHEAELKPLAQSHATKHKIP IKYLEFISEAIIHVLHSRHP GDFGADAQGAMNKALELFRK DIAAKYKELGYQG

Data sets:
Data typeCount
1H chemical shifts294
13C chemical shifts445
15N chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1apoMb1

Entities:

Entity 1, apoMb 153 residues - Formula weight is not available

1   VALLEUSERGLUGLYGLUTRPGLNLEUVAL
2   LEUHISVALTRPALALYSVALGLUALAASP
3   VALALAGLYHISGLYGLNASPILELEUILE
4   ARGLEUPHELYSSERHISPROGLUTHRLEU
5   GLULYSPHEASPARGPHELYSHISLEULYS
6   THRGLUALAGLUMETLYSALASERGLUASP
7   LEULYSLYSHISGLYVALTHRVALLEUTHR
8   ALALEUGLYALAILELEULYSLYSLYSGLY
9   HISHISGLUALAGLULEULYSPROLEUALA
10   GLNSERHISALATHRLYSHISLYSILEPRO
11   ILELYSTYRLEUGLUPHEILESERGLUALA
12   ILEILEHISVALLEUHISSERARGHISPRO
13   GLYASPPHEGLYALAASPALAGLNGLYALA
14   METASNLYSALALEUGLULEUPHEARGLYS
15   ASPILEALAALALYSTYRLYSGLULEUGLY
16   TYRGLNGLY

Samples:

sample_1: unfolded myoglobin, [U-95% 13C; U-95% 15N], 0.2 mM

Ex-cond-1: pH: 2.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
high field was required for maximum sensitivity for low concentration samples and forsample_1not availableEx-cond-1
maximum proton dispersionsample_1not availableEx-cond-1

Software:

FELIX v97 - transform and plot; data tables assignments were done by hand

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

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PDB
DBJ BAF03579
GB AAA72199
PRF 742482A
REF NP_001277651
SP P02185