BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4287

Title: 1H and 15N Chemical Shift Assignments of the Unstructured Yeast Target Membrane SNARE Sso1.

Authors: Fiebig, Klaus; Brunger, Axel

Citation: Fiebig, Klaus; Rice, Luke; Pollock, Elizabeth; Brunger, Axel. "Folding Intermediates of SNARE Complex Assembly"  Nat. Struct. Biol. 6, 117-123 (1999).

Assembly members:
Sso1, polymer, 268 residues, Formula weight is not available

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Sso1: GSHMSYNNPYQLETPFEESY ELDEGSSAIGAEGHDFVGFM NKISQINRDLDKYDHTINQV DSLHKRLLTEVNEEQASHLR HSLDNFVAQATDLQFKLKNE IKSAQRDGIHDTNKQAQAEN SRQRFLKLIQDYRIVDSNYK EENKEQAKRQYMIIQPEATE DEVEAAISDVGGQQIFSQAL LNANRRGEAKTALAEVQARH QELLKLEKSMAELTQLFNDM EELVIEQQENVDVIDKNVED AQLDVEQGVGHTDKAVKSAR KARKNKIR

Data sets:
Data typeCount
15N chemical shifts76
1H chemical shifts430

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sso11

Entities:

Entity 1, Sso1 268 residues - Formula weight is not available

1   GLYSERHISMETSERTYRASNASNPROTYR
2   GLNLEUGLUTHRPROPHEGLUGLUSERTYR
3   GLULEUASPGLUGLYSERSERALAILEGLY
4   ALAGLUGLYHISASPPHEVALGLYPHEMET
5   ASNLYSILESERGLNILEASNARGASPLEU
6   ASPLYSTYRASPHISTHRILEASNGLNVAL
7   ASPSERLEUHISLYSARGLEULEUTHRGLU
8   VALASNGLUGLUGLNALASERHISLEUARG
9   HISSERLEUASPASNPHEVALALAGLNALA
10   THRASPLEUGLNPHELYSLEULYSASNGLU
11   ILELYSSERALAGLNARGASPGLYILEHIS
12   ASPTHRASNLYSGLNALAGLNALAGLUASN
13   SERARGGLNARGPHELEULYSLEUILEGLN
14   ASPTYRARGILEVALASPSERASNTYRLYS
15   GLUGLUASNLYSGLUGLNALALYSARGGLN
16   TYRMETILEILEGLNPROGLUALATHRGLU
17   ASPGLUVALGLUALAALAILESERASPVAL
18   GLYGLYGLNGLNILEPHESERGLNALALEU
19   LEUASNALAASNARGARGGLYGLUALALYS
20   THRALALEUALAGLUVALGLNALAARGHIS
21   GLNGLULEULEULYSLEUGLULYSSERMET
22   ALAGLULEUTHRGLNLEUPHEASNASPMET
23   GLUGLULEUVALILEGLUGLNGLNGLUASN
24   VALASPVALILEASPLYSASNVALGLUASP
25   ALAGLNLEUASPVALGLUGLNGLYVALGLY
26   HISTHRASPLYSALAVALLYSSERALAARG
27   LYSALAARGLYSASNLYSILEARG

Samples:

sample_one: Sso1, [U-99% 15N], 1.2 mM

sample_conditions: ionic strength: 220; pH: 4.5; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
1H-15N-HSQCsample_onenot availablesample_conditions
1H-15N-NOESY-HSQCsample_onenot availablesample_conditions
1H-15N-TOCSY-HSQCsample_onenot availablesample_conditions

Software:

FELIX v95 - transformation

XEasy v1.3.11 - Assignment

NMR spectrometers:

  • Varian . 600 MHz

Related Database Links:

PDB
DBJ GAA26746
EMBL CAA47959 CAA54217 CAA64255 CAA97949 CAY86728
GB AHY77951 AJP41919 AJU23363 AJU24051 AJU24725
REF NP_015092
SP P32867
TPG DAA11204