BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4286

Title: 1H and 15N Chemical Shift Assignments of the Unstructured Yeast Vesicular SNARE Snc1.

Authors: Fiebig, Klaus; Pollock, Elizabeth; Brunger, Axel

Citation: Fiebig, Klaus; Rice, Luke; Pollock, Elizabeth; Brunger, Axel. "Folding Intermediates of SNARE Complex Assembly"  Nat. Struct. Biol. 6, 117-123 (1999).

Assembly members:
Snc1, polymer, 98 residues, Formula weight is not available

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Snc1: GSPEFMSSSTPFDPYALSEH DEERPQNVQSKSRTAELQAE IDDTVGIMRDNINKVAERGE RLTSIEDKADNLAVSAQGFK RGANRVRKAMWYKDLKMK

Data sets:
Data typeCount
15N chemical shifts92
1H chemical shifts512

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Snc11

Entities:

Entity 1, Snc1 98 residues - Formula weight is not available

1   GLYSERPROGLUPHEMETSERSERSERTHR
2   PROPHEASPPROTYRALALEUSERGLUHIS
3   ASPGLUGLUARGPROGLNASNVALGLNSER
4   LYSSERARGTHRALAGLULEUGLNALAGLU
5   ILEASPASPTHRVALGLYILEMETARGASP
6   ASNILEASNLYSVALALAGLUARGGLYGLU
7   ARGLEUTHRSERILEGLUASPLYSALAASP
8   ASNLEUALAVALSERALAGLNGLYPHELYS
9   ARGGLYALAASNARGVALARGLYSALAMET
10   TRPTYRLYSASPLEULYSMETLYS

Samples:

sample_one: Snc1, [U-99% 15N], 1.2 mM

sample_conditions: ionic strength: 220; pH: 4.5; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
1H-15N-HSQCsample_onenot availablesample_conditions
1H-15N-NOESY-HSQCsample_onenot availablesample_conditions
1H-15N-TOCSY-HSQCsample_onenot availablesample_conditions

Software:

FELIX v95 - transformation

XEasy v1.3.11 - Assignment

NMR spectrometers:

  • Varian . 600 MHz

Related Database Links:

PDB
DBJ GAA21395
EMBL CAY77620
GB AAA35069 AAC05002 AHY74337 AJO92283 AJO92361
REF NP_009372
SP P31109
TPG DAA06958