BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4108

Title: Acid/Urea denatured cold shock protein A (CspA)

Authors: Alexandrescu, Andrei; Rathgeb-Szabo, K.

Citation: Alexandrescu, Andrei; Rathgeb-Szabo, K.. "NMR Assignments for Acid-Denatured Cold Shock Protein A"  J. Biomol. NMR 11, 461-462 (1998).

Assembly members:
Cold shock protein A, polymer, 70 residues, 7400 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Cold shock protein A: MSGKMTGIVKWFNADKGFGF ITPDDGSKDVFVHFSAIQND GYKSLDEGQKVSFTIESGAK GPAAGNVTSL

Data typeCount
13C chemical shifts138
15N chemical shifts72
1H chemical shifts72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CspA1

Entities:

Entity 1, CspA 70 residues - 7400 Da.

1   METSERGLYLYSMETTHRGLYILEVALLYS
2   TRPPHEASNALAASPLYSGLYPHEGLYPHE
3   ILETHRPROASPASPGLYSERLYSASPVAL
4   PHEVALHISPHESERALAILEGLNASNASP
5   GLYTYRLYSSERLEUASPGLUGLYGLNLYS
6   VALSERPHETHRILEGLUSERGLYALALYS
7   GLYPROALAALAGLYASNVALTHRSERLEU

Samples:

sample_one: Cold shock protein A, [U-15N;U-13C], 2 mM; Urea 6 M

sample_conditions_one: pH: 2.7; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity+600 600 MHz

Related Database Links:

BMRB 4107 4296
PDB
DBJ BAB37864 BAE77739 BAG79354 BAH65698 BAI28199
EMBL CAD07979 CAP78016 CAQ33874 CAQ91030 CAR00519
GB AAA23617 AAB18533 AAB66357 AAB69447 AAC06036
PIR AG0981
REF NP_312468 NP_418012 NP_458276 NP_462550 NP_709333
SP P0A9X9 P0A9Y0 P0A9Y1 P0A9Y2 P0A9Y3