BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25501

Title: Backbone chemical shift assignments for the folded/unfolded drkN SH3 protein at pH 7.2   PubMed: 26195753

Authors: Lee, Jung Ho; Zhang, Dongyu; Hughes, Christopher; Okuno, Yusuke; Sekhar, Ashok; Cavagnero, Silvia

Citation: Lee, Jung Ho; Zhang, Dongyu; Hughes, Christopher; Okuno, Yusuke; Sekhar, Ashok; Cavagnero, Silvia. "Heterogeneous binding of the SH3 client protein to the DnaK molecular chaperone"  Proc. Natl. Acad. Sci. U. S. A. 112, 4206-4215 (2015).

Assembly members:
drkN_SH3, polymer, 59 residues, Formula weight is not available

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
drkN_SH3: MEAIAKHDFSATADDELSFR KTQILKILNMEDDSNWYRAE LDGKEGLIPSNYIEMKNHD

Data typeCount
13C chemical shifts293
15N chemical shifts110
1H chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1drkN SH3, native1
2drkN SH3, unfolded1

Entities:

Entity 1, drkN SH3, native 59 residues - Formula weight is not available

1   METGLUALAILEALALYSHISASPPHESER
2   ALATHRALAASPASPGLULEUSERPHEARG
3   LYSTHRGLNILELEULYSILELEUASNMET
4   GLUASPASPSERASNTRPTYRARGALAGLU
5   LEUASPGLYLYSGLUGLYLEUILEPROSER
6   ASNTYRILEGLUMETLYSASNHISASP

Samples:

sample_1: drkN SH3, [U-99% 13C; U-99% 15N], 0.3 mM; TRIS 50 mM; MgCl2 5 mM; KCl 50 mM; H2O 95%; D2O 5%

sample_2: drkN SH3, [U-99% 13C; U-99% 15N], 0.3 mM; TRIS 50 mM; MgCl2 5 mM; KCl 50 mM; H2O 95%; D2O 5%

sample_condition: pH: 7.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC 1sample_1isotropicsample_condition
3D CBCA(CO)NH 1sample_1isotropicsample_condition
3D HNCACB 1sample_1isotropicsample_condition
3D HNCO 1sample_1isotropicsample_condition
3D (HCA)CO(CA)NH 1sample_1isotropicsample_condition
2D 1H-15N HSQC 2sample_2isotropicsample_condition
3D CBCA(CO)NH 2sample_2isotropicsample_condition
3D HNCACB 2sample_2isotropicsample_condition
3D HNCO 2sample_2isotropicsample_condition
3D (HCA)CO(CA)NH 2sample_2isotropicsample_condition

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Agilent INOVA 600 MHz

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