BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25236

Title: ssNMR structure of a designed rocker protein   PubMed: 25525248

Authors: Wang, Tuo; Joh, Nathan; Wu, Yibing; DeGrado, William; Hong, Mei

Citation: Joh, Nathan; Wang, Tuo; Bhate, Manasi; Acharya, Rudresh; Wu, Yibing; Grabe, Michael; Hong, Mei; Grigoryan, Gevorg; DeGrado, William. "De novo design of a transmembrane Zn(II) transporting four-helix bundle"  Science 346, 1520-1524 (2014).

Assembly members:
entity_1, polymer, 26 residues, 2973.451 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: YYKEIAHALFSALXALSELY IAVRYX

Data sets:
Data typeCount
19F chemical shifts1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1_11
2entity_1_21
3entity_1_31
4entity_1_41

Entities:

Entity 1, entity_1_1 26 residues - 2973.451 Da.

1   TYRTYRLYSGLUILEALAHISALALEUPHE
2   SERALALEUPFFALALEUSERGLULEUTYR
3   ILEALAVALARGTYRNH2

Samples:

sample_1: entity_1, F19 on Phe 14, 1.5 mg

sample_conditions_1: temperature: 220 K; pH: 7; pressure: 1 atm; ionic strength: 0.02 M

Experiments:

NameSampleSample stateSample conditions
19F CODEXsample_1solidsample_conditions_1

Software:

pymol, Warren DeLano - modeling

NMR spectrometers:

  • Bruker DSX 400 MHz

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