BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25178

Title: Three-dimensional structure of the p7 protein of hepatitis C virus in phospholipid bilayers by solid-state NMR   PubMed: 23841474

Authors: Cook, Gabriel; Dawson, Lindsay; Tian, Ye; Das, Bibhuti; Opella, Stanley

Citation: Cook, Gabriel; Dawson, Lindsay; Tian, Ye; Opella, Stanley. "Three-dimensional structure of the p7 protein of hepatitis C virus in phospholipid bilayers by solid-state NMR"  Biochemistry ., .-..

Assembly members:
entity, polymer, 63 residues, 6693.997 Da.

Natural source:   Common Name: Hepatitis C virus   Taxonomy ID: 420174   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: ALENLVVLNAASVAGAHGIL SFLVFFSAAWYIKGRLAPGA AYAFYGVWPLLLLLLALPPR AYA

Data sets:
Data typeCount
13C chemical shifts86
15N chemical shifts47

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 63 residues - 6693.997 Da.

1   ALALEUGLUASNLEUVALVALLEUASNALA
2   ALASERVALALAGLYALAHISGLYILELEU
3   SERPHELEUVALPHEPHESERALAALATRP
4   TYRILELYSGLYARGLEUALAPROGLYALA
5   ALATYRALAPHETYRGLYVALTRPPROLEU
6   LEULEULEULEULEUALALEUPROPROARG
7   ALATYRALA

Samples:

sample_1: p7, [U-100% 13C; U-100% 15N], 3 – 4 mg; HEPES 20 mM; H2O 100%; DMPC 25 mg

sample_conditions_1: pH: 7.3; temperature: 298 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D NCACXsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1
2D 15N-13C HETCORsample_1isotropicsample_conditions_1
2D 13C/13C Correlationsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Xplor-NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

CS-Rosetta, Shen, Vernon, Baker and Bax - structure solution

NMR spectrometers:

  • Varian INOVA 700 MHz

Related Database Links:

BMRB 18863 25181
PDB
DBJ BAA01583 BAA02756
EMBL CAA43792
GB AAC15722 AAC15723 AAC15724 AAC15725 AAC15726
SP O92972