BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25004

Title: Chemical shift assignments for the parallel in-register D23N Iowa Mutant Beta Amyloid Fibrils   PubMed: 25543257

Authors: Sgourakis, Nikolaos; Qiang, Wei

Citation: Sgourakis, Nikolaos; Yau, Wai-Ming; Qiang, Wei. "Modeling an in-register, parallel "iowa" a beta fibril structure using solid-state NMR data from labeled samples with rosetta"  Structure 23, 216-227 (2015).

Assembly members:
D23N_Abeta, polymer, 26 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
D23N_Abeta: QKLVFFAENVGSNKGAIIGL MVGGVV

Data sets:
Data typeCount
13C chemical shifts59

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1D23N_Abeta1

Entities:

Entity 1, D23N_Abeta 26 residues - Formula weight is not available

1   GLNLYSLEUVALPHEPHEALAGLUASNVAL
2   GLYSERASNLYSGLYALAILEILEGLYLEU
3   METVALGLYGLYVALVAL

Samples:

D23N_parallel: D23N_Abeta, [U-100% 13C; U-100% 15N], 25 – 100 uM

sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: . atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C spin diffusionD23N_parallelsolidsample_conditions_1
1D 13C PITHIRDsD23N_parallelsolidsample_conditions_1
1D 13C-15N REDORD23N_parallelsolidsample_conditions_1

Software:

NA, NA - NA

NMR spectrometers:

  • Varian INOVA 400 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17774 18175
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