BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 21015

Title: solution structure of conotoxin pu14a   PubMed: 21968500

Authors: Zhang, Bingbing; Huang, Feijuan; Du, Weihong

Citation: Zhang, Bingbing; Huang, Feijuan; Du, Weihong. "Solution structure of a novel conotoxin with a distinctive loop spacing pattern"  Amino Acids ., .-. (2011).

Assembly members:
conotoxin pu14a, polymer, 19 residues, 2069.556 Da.

Natural source:   Common Name: Conus pulicarius   Taxonomy ID: 93154   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus pulicarius

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
conotoxin pu14a: DCPPHPVPGMHKCVCLKTC

Data sets:
Data typeCount
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1conotoxin pu14a1

Entities:

Entity 1, conotoxin pu14a 19 residues - 2069.556 Da.

1   ASPCYSPROPROHISPROVALPROGLYMET
2   HISLYSCYSVALCYSLEULYSTHRCYS

Samples:

sample_1: conotoxin pu14a 4 mM; TFA 0.01%; H2O 90%; D2O 10%

sample_2: conotoxin pu14a 4 mM; TFA 0.01%; D2O 100%

sample_conditions_1: pH: 3.0; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - processing

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - geometry optimization, structure solution

SPARKY v3.114, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

GB ACL13206 AIC77154
SP C6ZJQ2