BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 21014

Title: solution structure of conotoxin lt14a   PubMed: 21968500

Authors: Zhang, Bingbing; Huang, Feijuan; Du, Weihong

Citation: Zhang, Bingbing; Huang, Feijuan; Du, Weihong. "Solution structure of a novel conotoxin with a distinctive loop spacing pattern"  Amino Acids ., .-. (2011).

Assembly members:
conotoxin lt14a, polymer, 14 residues, 1395.745 Da.

Natural source:   Common Name: Conus litteratus   Taxonomy ID: 89445   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus litteratus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
conotoxin lt14a: MCPPLCKPSCTNCX

Data sets:
Data typeCount
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1conotoxin lt14a1

Entities:

Entity 1, conotoxin lt14a 14 residues - 1395.745 Da.

MCPPLCKPSCTNC(NH2)

1   METCYSPROPROLEUCYSLYSPROSERCYS
2   THRASNCYSNH2

Samples:

sample_1: conotoxin lt14a 4 mM; TFA 0.01%; H2O 90%; D2O 10%

sample_2: conotoxin lt14a 4 mM; TFA 0.01%; D2O 100%

sample_conditions_1: pH: 3.0; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - processing

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - geometry optimization, structure solution

SPARKY v3.0, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 400 MHz

Related Database Links: