BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 21008

Title: LPS bound structure of Temporin-1Tl   PubMed: 21586570

Authors: Bhattacharyya, Surajit

Citation: Bhunia, Anirban; Saravanan, Rathi; Mohanram, Harini; Mangoni, Maria; Bhattacharjya, Surajit. "NMR Structures and Interactions of Temporin-1Tl and Temporin-1Tb with Lipopolysaccharide Micelles: MECHANISTIC INSIGHTS INTO OUTER MEMBRANE PERMEABILIZATION AND SYNERGISTIC ACTIVITY"  J. Biol. Chem. 286, 24394-24406 (2011).

Assembly members:
entity, polymer, 13 residues, 3838.507 Da.

Natural source:   Common Name: common frog   Taxonomy ID: 8407   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rana temporaria

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
entity: FVQWFSKFLGRIL

Data sets:
Data typeCount
1H chemical shifts98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TemporinL, chain 11
2TemporinL, chain 21

Entities:

Entity 1, TemporinL, chain 1 13 residues - 3838.507 Da.

1   PHEVALGLNTRPPHESERLYSPHELEUGLY
2   ARGILELEU

Samples:

sample_1: TemporinL 0.5 mM; LPS 14.5 microM; H20 90%; D20 10%

sample_conditions_1: pH: 4.5; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

NMR spectrometers:

  • Bruker AMX 600 MHz

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