BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 20123

Title: NS4A(1-22)   PubMed: 18799730

Authors: Montserret, Roland; Penin, Francois

Citation: Brass, Volker; Berke, Jan Martin; Montserret, Roland; Blum, Hubert E.; Penin, Francois; Moradpour, Darius. "Structural determinants for membrane association and dynamic organization of the hepatitis C virus NS3-4A complex"  Proc. Natl. Acad. Sci. U.S.A. 105, 14545-14550 (2008).

Assembly members:
NS4A(1-22), polymer, 22 residues, Formula weight is not available

Natural source:   Common Name: Hepatitis C virus   Taxonomy ID: 11103   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C virus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
NS4A(1-22): STWVLVGGVLAALAAYCLST GS

Data sets:
Data typeCount
13C chemical shifts58
1H chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NS4A(1-22)1

Entities:

Entity 1, NS4A(1-22) 22 residues - Formula weight is not available

1   SERTHRTRPVALLEUVALGLYGLYVALLEU
2   ALAALALEUALAALATYRCYSLEUSERTHR
3   GLYSER

Samples:

sample_1: NS4A(1-22) 0.9 ± 0.05 mM; H2O 50%; trifluoroethanol 50%

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links:

BMRB 15580
DBJ BAA01515 BAA01582 BAA03581 BAI23205
EMBL CAC03609
GB AAA45534 AAA45676 AAA45677 AAB66324 AAB67036
REF NP_671491 NP_751925
SP P26664 P27958 Q03463 Q81754 Q913D4