BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20122

Title: Solution structure of CCAP-vil with one disulfide bond from Conus villepinii   PubMed: 20385188

Authors: Ohlenschlaeger, Oliver; Imhof, Diana; Miloslavina, Alesia

Citation: Miloslavina, Alesia; Ebert, Christina; Tietze, Daniel; Ohlenschlaeger, Oliver; Englert, Christoph; Goerlach, Matthias; Imhof, Diana. "An unusual peptide from Conus villepinii: synthesis, solution structure, and cardioactivity"  Peptides 31, 1292-1300 (2010).

Assembly members:
CCAP-vil, polymer, 10 residues, Formula weight is not available

Natural source:   Common Name: Villepin   Taxonomy ID: 257347   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Metazoa

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
CCAP-vil: PFCNSFGCYN

Data sets:
Data typeCount
1H chemical shifts57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CCAP-vil1

Entities:

Entity 1, CCAP-vil 10 residues - Formula weight is not available

1   PROPHECYSASNSERPHEGLYCYSTYRASN

Samples:

sample_1: CCAP-vil 2.9 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

XEASY, Bartels et al. - chemical shift assignment, data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

NMR spectrometers:

  • Bruker AvanceIII 750 MHz

Related Database Links: