BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20112

Title: CHARACTERIZATION OF THE LIGAND-FREE CONSTITUTIVE ACTIVITY OF THE THROMBOXANE A2 RECEPTOR BY THE INTEGRATED APPROACHES OF NMR SPECTROSCOPY AND A RECOMBINANT RECEPTOR   PubMed: 20590159

Authors: Chillar, Annirudha; Wu, Jiaxin; Cervantes, Vanessa; Ruan, Ke-He

Citation: Chillar, Annirudha; Wu, Jiaxin; Cervantes, Vanessa; Ruan, Ke-He. "Structural and functional analysis of the C-terminus of Galphaq in complex with the human thromboxane A2 receptor provides evidence of constitutive activity"  Biochemistry 49, 6365-6374 (2010).

Assembly members:
Gaq-Ct_peptide, polymer, 15 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Gaq-Ct_peptide: KDTILQLNLKEYNLV

Data sets:
Data typeCount
1H chemical shifts112
conformer coordinate sets1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Caq-ct1

Entities:

Entity 1, Caq-ct 15 residues - Formula weight is not available

1   LYSASPTHRILELEUGLNLEUASNLEULYS
2   GLUTYRASNLEUVAL

Samples:

sample_1: (Gaq-Ct peptide) 2.2 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

FELIX v2000, Accelrys Software Inc. - chemical shift assignment, chemical shift calculation, data analysis, peak picking, processing, refinement, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 20109 20118
EMBL CAG08422
GB ACF47673 ACF47675 EGW10015 EGW13773 EHB02791
REF XP_004048208 XP_004059801 XP_005431300 XP_005642455 XP_008508489