BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20110

Title: Solution structure of PV1-VPgpU   PubMed: 20441784

Authors: Schein, Catherine; Oezguen, Numan

Citation: Schein, Catherine; Oezguen, Numan; Noort, Gerbrand; Filippov, Dmitri; Paul, Aniko; Kumar, Eric; Braun, Werner. "NMR solution structure of poliovirus uridylyated peptide linked to the genome (VPgpU)"  Peptides 31, 1441-1448 (2010).

Assembly members:
VPgpU, polymer, 22 residues, Formula weight is not available

Natural source:   Common Name: Human enterovirus C   Taxonomy ID: 138950   Superkingdom: not available   Kingdom: Viruses   Genus/species: not available Enterovirus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
VPgpU: GAXTGLPNKKPNVPTIRTAK VQ

Data sets:
Data typeCount
15N chemical shifts1
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1VPgpU1

Entities:

Entity 1, VPgpU 22 residues - Formula weight is not available

Chemical conjugate of UMP and VPg

1   GLYALATYUTHRGLYLEUPROASNLYSLYS
2   PROASNVALPROTHRILEARGTHRALALYS
3   VALGLN

Samples:

sample_1: VPgpU 2.3 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.5; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

NOAH, Werner Braun group - peak picking

DIAMOD, Werner Braun group - structure solution

NMR spectrometers:

  • Varian UnityPlus 750 MHz

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