BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 20107

Title: NMR structure of conotoxin ImI analogue   PubMed: 19901032

Authors: Clark, Richard; Armishaw, Chris

Citation: Armishaw, Christopher; Singh, Narender; Medina-Franco, Jose; Clark, Richard; Scott, Krystle; Houghten, Richard; Jensen, Anders. "A synthetic combinatorial strategy for developing alpha-conotoxin analogs as potent alpha7 nicotinic acetylcholine receptor antagonists."  J. Biol. Chem. 285, 1809-1821 (2010).

Assembly members:
conotoxin ImI analogue, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
conotoxin ImI analogue: GCCSDPRCLYXC

Data sets:
Data typeCount
1H chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1conotoxin ImI analogue1

Entities:

Entity 1, conotoxin ImI analogue 12 residues - Formula weight is not available

residue 11 is an aminobutyric acid, C-terminus is amidated

1   GLYCYSCYSSERASPPROARGCYSLEUTYR
2   ABACYS

Samples:

sample_1: [A9L, W10Y, R11Aba]ImI 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 3.5; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CNS v2.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

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