BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 20105

Title: BI-32169   PubMed: 20043911

Authors: Knappe, Thomas A.; Linne, Uwe; Xie, Xiulan; Marahiel, Mohamed A.

Citation: Knappe, Thomas; Linne, Uwe; Xie, Xiulan; Marahiel, Mohamed. "The glucagon receptor antagonist BI-32169 constitutes a new class of lasso peptides."  FEBS Lett. 584, 785-789 (2010).

Assembly members:
BI-32169, polymer, 19 residues, Formula weight is not available

Natural source:   Common Name: Streptomyces sp.   Taxonomy ID: 1931   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptomyces Streptomyces sp.

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
BI-32169: GLPWGCPSDIPGWNTPWAC

Data sets:
Data typeCount
1H chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BI1

Entities:

Entity 1, BI 19 residues - Formula weight is not available

1   GLYLEUPROTRPGLYCYSPROSERASPILE
2   PROGLYTRPASNTHRPROTRPALACYS

Samples:

sample_1: BI-321693 – 5 mM

sample_conditions_1: pH: .; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Goddard, Guntert, Mumenthaler and Wuthrich, Tripos - chemical shift assignment, geometry optimization, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

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