BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 20104

Title: structural studies of antimicrobial peptide XT-7 and its Lysine mutated peptide   PubMed: 20116461

Authors: Subasinghage, Anusha; Hewage, Chandralal; Conlon, Michael

Citation: Subasinghage, Anusha; Conlon, Michael; Hewage, Chandralal. "Development of potent anti-infective agents from Silurana tropicalis: Conformational analysis of the amphipathic, alpha-helical antimicrobial peptide XT-7 and its non-haemolytic analogue [G4K]XT-7"  Biochim. Biophys. Acta 1804, 1020-1028 (2010).

Assembly members:
XT-7, polymer, 18 residues, Formula weight is not available

Natural source:   Common Name: frog   Taxonomy ID: 8364   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus tropicalis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
XT-7: GLLGPLLKIAAKVGSNLL

Data sets:
Data typeCount
1H chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1XT-7 peptde1

Entities:

Entity 1, XT-7 peptde 18 residues - Formula weight is not available

1   GLYLEULEUGLYPROLEULEULYSILEALA
2   ALALYSVALGLYSERASNLEULEU

Samples:

sample_1: XT-7 2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 3.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
1Dsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY v3.11, Goddard - chemical shift assignment, peak picking

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - chemical shift calculation, structure solution

SYBYL v6.8.1, Tripos - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

SP P84381