BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 20102

Title: C-Terminal helix of the SERCA2b Ca2+ pump (ATP2A2B)   PubMed: 19846779

Authors: Vandecaetsbeek, Ilse; Trekels, Mieke; De Maeyer, Marc; Ceulemans, Hugo; Lescrinier, Eveline; Raeymaekers, Luc; Wuytack, Frank; Vangheluwe, Peter

Citation: Vandecaetsbeek, Ilse; Trekels, Mieke; De Maeyer, Marc; Ceulemans, Hugo; Lescrinier, Eveline; Raeymaekers, Luc; Wuytack, Frank; Vangheluwe, Peter. "Structural basis for the high Ca2+ affinity of the ubiquitous SERCA2b Ca2+ pump."  Proc. Natl. Acad. Sci. U.S.A. 106, 18533-18538 (2009).

Assembly members:
t11, polymer, 20 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
t11: DGISWPFVLLIMPLVIWVYS

Data sets:
Data typeCount
1H chemical shifts133

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1t111

Entities:

Entity 1, t11 20 residues - Formula weight is not available

1   ASPGLYILESERTRPPROPHEVALLEULEU
2   ILEMETPROLEUVALILETRPVALTYRSER

Samples:

sample_1: t11 3 mM; CDCL3 50%; DMSO 50%

sample_conditions_1: pH: 7; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, torsion angle dynamics

TOPSPIN, Bruker Biospin - collection

CARA, Rochus Keller, Kurt W thrich - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

DBJ BAG57266
EMBL CAA33170 CAA34825 CAA37784 CAJ42045 CAJ42886
GB AAA53193 AAH35588 EAW97902 EAW97905 EAW97906
REF NP_001075234 NP_733765 NP_999030 XP_002798822 XP_004276771
SP P11607 P16615