BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 20082

Title: Apelin17 bound to SDS micelles   PubMed: 19708686

Authors: Langelaan, David; Rainey, Jan

Citation: Langelaan, David; Rainey, Jan. "Headgroup-Dependent Membrane Catalysis of Apelin-Receptor Interactions Is Likely"  J. Phys. Chem. B. 113, 10465-10471 (2009).

Assembly members:
Apelin-17, polymer, 17 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Apelin-17: KFRRQRPRLSHKGPMPF

Data sets:
Data typeCount
13C chemical shifts64
15N chemical shifts13
1H chemical shifts146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Apelin-171

Entities:

Entity 1, Apelin-17 17 residues - Formula weight is not available

1   LYSPHEARGARGGLNARGPROARGLEUSER
2   HISLYSGLYPROMETPROPHE

Samples:

sample_1: Apelin-17 0.5 mM; D2O 10%; sodium acetate 20 mM; Sodium Azide 1 mM; DSS 1 mM; Sodium Dodecyl Sulfate, [U-99% 2H], 81 mM

sample_conditions_1: pH: 5.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Bruker Avance 500 MHz
  • Bruker Avance 700 MHz

Related Database Links:

BMRB 16275 20029 20030 20031
DBJ BAA84974 BAA84975 BAA84976 BAA84977 BAC31156
EMBL CAC83678
GB AAF25814 AAF25815 AAH20015 AAH21104 AAH80843
REF NP_001177797 NP_038940 NP_059109 NP_113800 NP_776928
SP Q9R0R3 Q9R0R4 Q9TUI9 Q9ULZ1
TPG DAA13389