BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20076

Title: NMR Solution Structure of a TC5b_D9E miniprotein   PubMed: 18161949

Authors: Hudaky, Peter; Straner, Pal; Rovo, Petra

Citation: Hudaky, Peter; Straner, Pal; Farkas, Viktor; Varadi, Gyorgyi; Toth, Gabor; Perczel, Andras. "Cooperation between a Salt Bridge and the Hydrophobic Core Triggers Fold Stabilization in a Trp-Cage Miniprotein"  Biochemistry 47, 1007-1016 (2008).

Assembly members:
TC6b, polymer, 20 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
TC6b: NLYIQWLKEGGPSSGRPPPS

Data sets:
Data typeCount
1H chemical shifts134

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TC6b1

Entities:

Entity 1, TC6b 20 residues - Formula weight is not available

1   ASNLEUTYRILEGLNTRPLEULYSGLUGLY
2   GLYPROSERSERGLYARGPROPROPROSER

Samples:

sample_1: TC6b 2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.61; temperature: 282 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, structure solution

SPARKY, Goddard - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

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