BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20075

Title: Solution structure of the N(1-21)MeuTXK-beta in presence of TFE   PubMed: 20045493

Authors: Zhu, Shun-Yi; Gao, Bin; Aumelas, Andre; Rodriguez, Maria del Carmen; Lanz-Mendoza, Humberto; Peigneur, Steve; Diego-Garcia, Elia; Martin-Eauclaire, Marie-France; Tytgat, Jan; Possani, Lourival

Citation: Zhu, Shunyi; Gao, Bin; Aumelas, Andre; del Carmen Rodriguez, Maria; Lanz-Mendoza, Humberto; Peigneur, Steve; Diego-Garcia, Elia; Martin-Eauclaire, Marie-France; Tytgat, Jan; Possani, Lourival. "MeuTXKbeta1, a scorpion venom-derived two-domain potassium channel toxin-like peptide with cytolytic activity."  Biochim. Biophys. Acta 1804, 872-883 (2010).

Assembly members:
MeuTXKbeta1, polymer, 21 residues, Formula weight is not available

Natural source:   Common Name: lesser Asian scorpion   Taxonomy ID: 34648   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesobuthus eupeus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
MeuTXKbeta1: GFREKHFQRFVKYAVPESTL R

Data sets:
Data typeCount
1H chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MeuTXKbeta11

Entities:

Entity 1, MeuTXKbeta1 21 residues - Formula weight is not available

1   GLYPHEARGGLULYSHISPHEGLNARGPHE
2   VALLYSTYRALAVALPROGLUSERTHRLEU
3   ARG

Samples:

sample_1: MeuTXKbeta11.5 – 2.0 mM

sample_conditions_1: pH: 4.5; temperature: 286 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection, processing

DYANA, Guntert, Braun and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

GB ABP35518 ABP35519 ABR20127
SP A9XE59 A9XE60