BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 20051

Title: Biological and structural study of Obestatin and its analogues Structural importance of Obestatin and its biology   PubMed: 20553778

Authors: Subasinghage, Anusha; Hewage, Chandralal; Flatt, Peter; Green, Brian

Citation: Subasinghage, Anusha; Green, Brian; Flatt, Peter; Irwin, Nigel; Hewage, Chandralal. "Metabolic and structural properties of human obestatin {1-23} and two fragment peptides."  Peptides 31, 1697-1705 (2010).

Assembly members:
Human_Obestatin, polymer, 23 residues, 2547.9 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Human_Obestatin: FNAPFDVGIKLSGVQYQQHS QAL

Data sets:
Data typeCount
1H chemical shifts137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human Obestatin1

Entities:

Entity 1, Human Obestatin 23 residues - 2547.9 Da.

1   PHEASNALAPROPHEASPVALGLYILELYS
2   LEUSERGLYVALGLNTYRGLNGLNHISSER
3   GLNALALEU

Samples:

sample_1: Human Obestatin 1.5 mM

sample_conditions_1: pH: 3.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - processing

SPARKY v3.110, Goddard - peak picking

SYBYL v6.8.1, Tripos - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

DBJ BAA89371 BAB19045 BAG16755 BAG16756 BAG16757
EMBL CAB65733
GB AAG10300 AAH25791 AAQ74381 AAQ74837 AAQ89412
REF NP_001028075 NP_001128413 NP_001128416 NP_001128417 NP_001128418
SP A0MLS4 Q9UBU3