BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20050

Title: NMR STRUCTURE OF DPAKKR, A CYCLIC PENTAPEPTIDE MIMIC OF BDNF   PubMed: 18809686

Authors: Morton, Craig; Hughes, Richard

Citation: Fletcher, Jordan; Morton, Craig; Zwar, Richard; Murray, Simon; O'Leary, Paul; Hughes, Richard. "Design of a conformationally defined and proteolytically stable circular mimetic of brain-derived neurotrophic factor"  J. Biol. Chem. 283, 33375-33383 (2008).

Assembly members:
D-PAKKR, polymer, 5 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
D-PAKKR: XAKKR

Data sets:
Data typeCount
1H chemical shifts41

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1D-PAKKR monomer1

Entities:

Entity 1, D-PAKKR monomer 5 residues - Formula weight is not available

Residue 1 is a D-proline. The peptide is cyclised so that the C-terminus (Arg) links back to the N-terminus (D-Pro).

1   DPRALALYSLYSARG

Samples:

sample_1: D-PAKKR 2-5 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.2; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H ROESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

DYANA, Guntert, Braun and Wuthrich - structure solution

NMR spectrometers:

  • Varian INOVA 400 MHz

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