BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 20044

Title: NMR structure of the Interleukin-8 C-terminal domain in solution with SDS micelles   PubMed: 19813761

Authors: Bourbigot, Sarah; Booth, Valerie

Citation: Bourbigot, Sarah; Fardy, Liam; Waring, Alan; Yeaman, Michael; Booth, Valerie. "Structure of chemokine-derived antimicrobial Peptide interleukin-8alpha and interaction with detergent micelles and oriented lipid bilayers."  Biochemistry 48, 10509-10521 (2009).

Assembly members:
interleukin-8_C-terminal_domain, polymer, 19 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
interleukin-8_C-terminal_domain: KENWVQRVVEKFLKRAENS

Data sets:
Data typeCount
15N chemical shifts4
1H chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 19 residues - Formula weight is not available

1   LYSGLUASNTRPVALGLNARGVALVALGLU
2   LYSPHELEULYSARGALAGLUASNSER

Samples:

sample_1: interleukin-8 C-terminal domain, [U-15N]-Leu;[U-15N]-Ala;[U-15N]-Val, 1.5 mM; H2O 90%; D2O 10%; DSS 0.4 mM; sodium azide 0.4 mM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298.15 K

sample_conditions_2: pH: 5; pressure: 1 atm; temperature: 303.15 K

sample_conditions_3: pH: 5; pressure: 1 atm; temperature: 308.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_3
2D 1H-1H TOCSYsample_1isotropicsample_conditions_3
2D 1H-1H NOESYsample_1isotropicsample_conditions_3
2D 1H-1H TOCSYsample_1isotropicsample_conditions_2

Software:

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.110, Goddard - data analysis, peak picking

ProcheckNMR, Laskowski and MacArthur - data analysis

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

BMRB 280
PDB
DBJ BAA03245 BAG34815 BAJ20387
EMBL CAA68742 CAA77745 CAG46948
GB AAA35611 AAA36323 AAA59158 AAA74722 AAB01177
REF NP_000575 XP_001156432 XP_002814902 XP_002814903 XP_003832383
SP P10145