BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 20034

Title: Solution NMR Structure of 4F Associated With DMPC Disc   PubMed: 18845546

Authors: Mishra, Vinod

Citation: Mishra, Vinod; Palgunachari, Mayakonda; Krishna, N.; Glushka, John; Segrest, Jere; Anantharamaiah, G.. "Effect of leucine to phenylalanine substitution on the nonpolar face of a class a amphipathic helical peptide on its interaction with lipid: High-resolution solution nmr studies of 4F:Dimyristoylphosphatidylcholine discoidal complex"  J. Biol. Chem. 283, 34393-34402 (2008).

Assembly members:
4F, polymer, 19 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
4F: XDWFKAFYDKVAEKFKEAF

Data sets:
Data typeCount
1H chemical shifts137
conformer coordinate sets1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
14F Associated With DMPC Disc1

Entities:

Entity 1, 4F Associated With DMPC Disc 19 residues - Formula weight is not available

1   ACEASPTRPPHELYSALAPHETYRASPLYS
2   VALALAGLULYSPHELYSGLUALAPHE

Samples:

sample_1: sodium phosphate 5 mM

sample_conditions_1: pH: 5.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1

Software:

X-PLOR, Brunger - geometry optimization

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian INOVA 900 MHz

Related Database Links: