BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 20027

Title: Brome Mosaic Virus protein 1a Helix A bound to SDS micelle   PubMed: 19325881

Authors: Liu, Ling; Westler, William; Wang, Xiaofeng; Diaz, Arturo; Ahlquist, Paul

Citation: Liu, Ling; Westler, William; den Boon, Johan; Wang, Xiaofeng; Diaz, Arturo; Steinberg, H.; Ahlquist, Paul. "An amphipathic alpha-helix controls multiple roles of brome mosaic virus protein 1a in RNA replication complex assembly and function"  PLoS Pathog. 5, e1000351-e1000351 (2009).

Assembly members:
1a, polymer, 18 residues, 2088.469 Da.

Natural source:   Common Name: not available   Taxonomy ID: 12302   Superkingdom: Virus   Kingdom: not available   Genus/species: Brome mosaic

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
1a: VAFALTLNLYQKYEKLTA

Data sets:
Data typeCount
13C chemical shifts57
15N chemical shifts17
1H chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
11a1

Entities:

Entity 1, 1a 18 residues - 2088.469 Da.

1   VALALAPHEALALEUTHRLEUASNLEUTYR
2   GLNLYSTYRGLULYSLEUTHRALA

Samples:

sample_1: peptide, [U-100% 13C; U-100% 15N], 0.8 mM; SDS-d25 100 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

PISTACHIO, Eghbalnia, Bahrami, Wang, Assadi and Markley - chemical shift assignment

PECAN, Eghbalnia - Secondary structure

SPARKY, Goddard - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

VNMRJ, Varian - collection

ATHNOS-CANDID, Herrmann - chemical shift assignment, peak picking, structure solution

PSVS, Bhattacharya and Montelione - refinement, validation

TALOS, Cornilescu, Delaglio and Bax - data analysis

HIFI, Eghbalnia et al - collection

NMR spectrometers:

  • Varian DirectDrive 600 MHz

Related Database Links:

DBJ BAJ41520
EMBL CAA26228 CAA41361
GB ABF83485 ADW09019 AJY78069 AKA44627
REF NP_041196
SP P03588